XIV. STAINING SECTIONS WITH BISMAECK- 

 BEOWN, LOBPFLBR'S ALKALINE MBTHYL- 

 BNB-BLUB, OR WATERY SOLUTIONS OF 

 THE ANILINE DYES. 



1. Section from alcohol to water, one minute. 



2. Stain with Bismarck-brown,^ Loeffler's alkaline 

 methylene-blue solution,^ or with a watery solution of one 

 of the aniline dyes,' for ten minutes. 



3. Decolorize in a watery solution of acetic acid 

 (1 :1000), about one minute. 



4. Thoroughly wash in 96 per cent, alcohol, using 

 fresh alcohol several times until no more coloring matter 

 comes away. 



5. Oa, balsam, etc. 



iBismarck-brown does not stain the bacteria at the same time 

 and is only used to bring out the tissue-structure. 



2L0EFFLBR'S AliKALINB METHYLENE-BLUE. 

 Concentrated alcohol solution methylene-blue . ..30 cc 

 Watery solution potash (1:1000) : . . . .100 cc 



3See I, foot-note 2, a and b. 



43 



