but semm would also not stain in such a definite man- 

 ner; moreover, a similar membrane, though much less 

 marked, has been observed in cultures of this organism 

 upon some of our media. We must, therefore, consider this 

 membrane to be a true portion of the bacillus itself and it 

 gives the organism, in the blood of anthrax animals, a very 

 characteristic appearances (PL 11, Fig. 1), which may be 

 brought out by staining, as follows: 



1. Make the cover-glass preparation from the blood, 

 splenic-pulp, etc. 

 . 2. Dry and fix. 



3. Flood the glass (held in the forceps) with 2 per 

 cent, gentian-violet solution (See I, foot-note 2 a) and stain 

 from a quarter to a half minute. 



4. Wash in water. 



5. Decolorize in 1 to 2 per cent, acetic acid from five 

 to twenty seconds. 



6. Wash in water. 



7. Mount, etc. 



51 



