7. Imbed in paraflBn.' 



8. Out with a Minot microtome. Such sections may 

 be placed in a box and they will keep indefinitely. Be- 

 fore staining — 



9. Sections into chloroform or benzole to dissolve 

 parafQn, using two watch-glasses of the fluid. 



10. Into absolute alcohol, using two watch-glasses of 

 tlie fluid. 



11. Stain. 



iFor this purpose cheap and very satisfactory paraffin moulds 

 may be aade frcm four pieces of heavy type-metal (brass is 

 in commoner use, but is more expensive). These pieces 

 need not be fastened together, thus permitting moulds 

 of different sizes to be formed. Small pasteboard boxes 

 may also be used. These pieces of metal are placed upon a glass 

 plate, a mould of the desired size is formed and filled with melted 

 paraffin, first, however, smearing the surfaces, which are later to 

 come in contact with the paraffin, with the glycerin. Soon a thin, 

 semi-solid layer of paraffin forms at the bottom of the mould, and 

 the material to be cut is then placed into and held in any posilion de- 

 sired by this layer. When the paraffin has cooled so that a thin film 

 or crust has formed on its sutface, immerse the mould and all in 

 cold water. The block of paraffin is thus quickly hardened and the 

 mould can almost immediately be removed. 



61 



