CONTENTS. 



INTEODUCTION. 



PAGE 



The overthrow of the doctrine of spontaneous generation—" Omne 

 Tivum ex vivo"— Earlier bacteriological studies— The birth of mod- 

 ern bacteriology . 13-26 



CHAPTEE I. 



Definition of bacteria— Their place in nature— Difference between 

 parasites and saprophytes— Nutrition of bacteria— Products of bac- 

 teria—Their relation to oxygen— Influence of temperature upon 

 their growth . . . 27-35 



CHAPTER II. 



Morphology of bacteria — Grouping— Mode of multiplication— 

 Spore-formation — Motility . . . 36-46 



CHAPTE R III. 



Principles of sterilization by heat — Methods employed— Discon- 

 tinued sterilization— Sterilization under pressure — Apparatus em- 

 ployed — Chemical disinfection and sterilization . 47-71 



CHAPTER IV. 



Principles involved in the methods of isolation of bacteria in pure 

 culture by the plate method of Koch— Materials employed . 72-78 



CHAPTER V. 



Preparationof nutrient media— Bouillon, gelatin, agar-agar, potato, 

 blood-serum, etc. . . . 7'J-IOS 



