CONTENTS. 



CHAPTEE VI. 



PAGE 



Preparation ol the tubes, flasks, etc., in which the media are to 

 be preserved . . . . . 109-112 



CHAPTEE VII. 



Technique of making plates— Esmarch tubes, Petri plates, etc. . 113-124 



CHAPTEE VIII. 



The incubating-oven— Gas-pressure regulator— Thermo-regulator— 

 Safety burner employed in heating the incubator. . 12&-132 



CHAPTEE IX. 



The study of colonies— Their naked-eye peculiarities and their ap- 

 pearance under different conditions— Differences in the structure 

 of colonies of different species of bacteria— Stab-cultures— Slant- 

 cultures . . . 133-138 



CHAPTEE X. 



Methods of staining— Solutions employed- Preparation and stain- 

 ing of coyer-slips- Preparation of tissues for section-cutting— Stain- 

 ing of tissues— Special staining-methods . . . 139-176 



CHAPTEE XI. 



Systematic study of an organism— Points to be considered in iden- 

 tifying an organism as a definite species 177-205 



CHAPTEE XII. 



Inoculation ot animals — Subcutaneous inoculation ; intravenous 

 injection— Inoculation into the great serous cavities, and into the an- 

 terior chamber of the eye— Observation of animals after inoculation 206-227 



CHAPTEE XIII. 



Post-mortem examination of animals — Bacteriological examina- 

 tion of the tissues — Disposal of tissues and disinfection of instru- 

 ments after the examination . 228-233 



