320 BACTERIOLOGY. 



nate with points at which it either does not stain at all 

 or only slightly. 



The animals that are susceptible to infection by this 

 organism are horses, asses, iield mice, guinea-pigs, and 

 cats. Baumgarten records cases of infection in lions 

 and tigers that have been fed, in menageries, with flesh 

 from horses affected with the disease. Rabbits are but 

 slightly susceptible; dogs and sheep still less so. Man 

 is susceptible, and infection not rarely terminates fatally. 

 White mice, common gray house-mice, rats, cattle, and 

 hogs are insusceptible. 



Inoculation Experiments. — The most favorable 

 animal upon which to study the pathogenic properties 

 of this organism in the laboratory is the common field 

 mouse. When inoculated subcutaneously with a small 

 portion of a pure culture of the glanders bacillus death 

 ensues in about seventy-two hours. The most conspicu- 

 ous tissue-changes will be enlargement o"f the spleen, 

 which is at the same time almost constantly studded 

 with minute gray nodules, the typical glanders nodule. 

 They are rarely present in the lungs, but may frequently 

 be seen in the liver. From these nodules the glanders 

 bacillus may be obtained in pure culture. With the 

 exception of the characteristic nodule, the disease as 

 seen in this animal presents none of the characteristics 

 that it displays in the horse and ass. The clinical and 

 pathological manifestations resulting from inoculation 

 of guinea-pigs are much more characteristic. The ani- 

 mal lives usually from six to eight weeks after inocu- 

 lation, and in this time becomes affected with a group 

 of most interesting and peculiar pathological processes. 

 The specific inflammatory condition of the mucous 

 membrane of the nostrils is almost always present. The 



