INTRODUCTION. 7 
sel as in the case of the jugular. Lift up the artery with 
the index finger (Fig. 4), and make an oblique incision 
with the scissors. The cannula with the connecting tube 
attached should then be inserted caudad in the oblique cut 
of the artery (Fig. 5), and the string 
s drawn tight so as to hold the cannula 
in place. The string s¢ should then 
be tightened to prevent the injection 
mass from running out where the can- 
-nula is inserted. After stirring the 
red mass, filling the syringe, and slip- 
ping the nozzle into the connecting 
tube the operator should press slowly 
but continuously on the plunger until 
that portion of the carotid craniad of 
the string st is well distended. In a 
large cat this will not occur until the 
: - E Fic. 5. MeEtTHop oF 
syringe is nearly or quite empty. INSERTING THE CANS 
When the vessels are full, the can- areas Ves- 
nula is withdrawn while the string s is 
tightened. The syringe is washed out, after which the blue 
mass is well stirred and injected caudad into the external 
jugular in the same manner as described for the arteries. 
By making a slit about three inches long in the abdominal 
wall, a fold of the intestine may be pulled out so that its 
lumen can be filled with about 200 c.c. of 15% formalin. 
The same amount should be injected into the lungs by 
inserting the cannula caudad into the ventral wall of the 
trachea. If the cat is not to be used at once, it may be 
preserved indefinitely in a jar of five per cent. formaline. 
The portal system is not injected through the jugular 
vein. The portal trunk just caudad of where it enters the 
liver may be found by cutting a piece from the abdominal 
wall on the right ventral aspect just caudad of the last rib. 
