8 ELEMENTS OF MAMMALIAN ANATOMY. 
After two loose knots are tied around this trunk as directed 
for the jugular, the cut is made and the cannula inserted 
caudad. About 15 c.c. of the blue mass may be injected 
into the portal system of a large specimen. This should 
not be injected until the other systems have been filled. 
The lymphatic system must likewise be injected for 
demonstration. While the cat is being anesthetized, 5 gm. 
of soluble Berlin blue are dissolved in 100 gm. of water, 
and the solution warmed to about the 
temperature of the body. As soon as 
the cat is unconscious the syringe should 
be warmed by filling it with hot water, 
and the point of the cannula pushed ob- 
liquely proximad under the thick skin on 
the palm (lig. 6) of the paw. The 
syringe after being half filled with Berlin 
Fic. 6 Parmar Plue solution is attached to the cannula 
Aspect or Cat's and the plunger pushed in very slowly 
Paw WITH Can- : 
NuLA Insertep, 80 that one-fourth of the amount in the 
syringe is forced out in ten minutes. 
While the injection is being made the limb should be gently 
massaged by pinching and rubbing from the foot toward 
the body. This facilitates the flow of the liquid in the 
lymph-vessels. In this manner the lymphatics of each limb 
are filled. The author has been able to fill the left thoracic 
duct by continuing the injection for about twenty minutes 
in the palm of the left paw. The lymphatics of the head 
and neck may be injected in a similar manner by inserting 
the cannula beneath the skin on the tip of the ear, the tip 
of the tongue, the tip of the nose, and the lips. 
In order to inject the lacteals the abdominal cavity must 
be opened, and the cannula inserted very obliquely into the 
wall of the small intestine so that its point is between the 
mucous and muscular coats. The half-filled syringe being 
