of Hawaii); the unfortunate circumstance that there was no possibility 

 of making drawings of the hving specimens also has lessened the value 

 of the researches carried out here. 



Better opportunities were found on the following places visited, the 

 Biological Station at Nanaimo, Vancouver, B. C. and at La JoUa, Cali- 

 fornia, where, of course, the usual laboratory facihties were available. 

 Several interesting forms were studied here, more or less completely. 

 Also at the next place, the Island of Taboga in the Bay of Panama, a rich 

 fauna was available for study, and fertihzation of quite a number of species 

 was undertaken; the results were, hovewer, not so good as desirable here, 

 the larvae generally dying off before the full shape had been acquired. 

 This was doubtless due to the water being not pure enough there near 

 the great Canal and all the traffic. 



While staying here at Taboga I had the great pleasure of being invited 

 to partake in the Carnegie Expedition to Tobago, B. W. I, (near Trinidad). 

 In the temporary laboratory established here close to the coral reef at 

 Buccoo Bay excellent results were obtained, the development of ten dif- 

 ferent species being studied more or less completely during the stay of 

 only four weeks. — On leaving this place I undertook again to carry some 

 of the cultures along with me, namely of Tropiometra carinata and Echino- 

 metra lucunter, the first in the Pentacrinoid stage, the second just meta- 

 morphosed. While the first of these was too much chilled during one 

 cold night near New York, the latter stood the transport and, after having 

 been kept for some weeks in the New York Aquarium was again taken 

 onboard the steamer to Europe and taken to Copenhagen. Some few 

 of the specimens were still alive and in fairly good condition on the arrival 

 to Copenhagen and thus had stood the transport from the Westindies to 

 Denmark under conditions not altogether favourable. 



The successful experiments made with such transports of larval cultures, 

 as upon the whole the several successful rearings of larvae under very 

 adverse conditions give an indication of their often surprising hardiness 

 and open the view to far reaching experiments. 



It is well known to everybody, who has undertaken to rear Echinoderm 

 larvae, how this task has been faciUtated to a very high degree through the 

 work of Allen and Nelsoni), the pure cultures of the Diatom Nitzschia 

 closterium, forma tenuissima obtained by them being in many cases very 

 successfully used as food for the Echinoderm larvse. When starting on 

 my voyage I had a small sample of such culture sent to me from Plymouth. 

 Unfortunately, however, it was all dead, before I could arrange for rais- 



1) C. J. Allen and E. W. Nelson. On the artificial culture of marine Plankton organisms 

 Quart. Journ. Micr. Science. Vol. 55. 1910. lai^Lon organisms. 



