CONTENTS 



List of Text and Reference Books 

 Apparatus and Material 

 Laboratory Maxims 



Page 

 xi 



xiii 



XV 



Exercises 



I. Cleaning glassware 



II. Plugging test tubes and flasks and sterilizing the glass 

 ware 



III. The preparation of bouillon 



IV. The preparation of agar and gelatin 



V. Inoculating tubes of bouillon, agar, and gelatin 



VI. The examination of cultures 



VII. Making and staining cover-glass preparations and for- 

 mulae for staining solutions 

 VIII. Making plate and Esmarch roll cultures 

 IX. The examination of plate cultures and the making of 



subcultures from colonies 



X. The preparation of certain special media 

 XI. Inoculating special media and examining cultures 

 XII. The examination of cultures on special media 



XIII. The examination of cultures (continued) 



XIV. The classification of bacteria .... 

 XV. The morphology of streptococcus and micrococcus 



XVI. The morphology of micrococcus (staphylococcus form) 



and sarcina 



XVII. The morphology of bacillus . 

 XVIII. The morphology of bacterium and spirillum 



XIX. Staining spores 



XX. Staining the flagella on motile bacteria . 

 XXI. Staining tubercle bacteria (bacilli) 

 XXII. Making cultures of anaerobic bacteria . 

 XXIII. Examination of cultures of ariaerobic bacteria 

 vii 



5 



7 



II 



14 

 17 



21 

 27 



30 

 33 

 37 

 39 

 41 

 42 



45 



47 

 48 



49 

 50 



52 

 55 

 57 

 59 



