XVI LABORATORY MAXIMS 



8. If by accident, a drop or more of a culture should be 

 spilled upon the table or floor, pour over it a sufficient quan- 

 tity of a disinfectant (corrosive sublimate solution i-iooo, or a 

 5% solution of carbolic acid) to completely cover the infected 

 area. After this has acted for ten minutes wipe it up and boil 

 or burn the cotton or cloth. If any of the culture should drop 

 on the hands or clothing, a disinfectant should be applied 

 immediately. 



9. In sterilizing culture media, always see that there is 

 enough water in the pan of the steam sterilizer or in the water 

 bath before hghting the gas. Do not put the media in a 

 sterilizer and leave the laboratory. 



10. Always disinfect, by boiUng, all cultures before cleaning 

 the tubes or plates containing them. (A liberal supply of 

 cleaning mixture can be used to advantage in some instances 

 for destroying cultures.) 



11. At the beginning of each laboratory session read the 

 directions for the next exercise in order to be able to make 

 any preliminary preparations which may be required. 



12. Careful notes should be taken on all observations made 

 in the study of cultures and preparations made from them. 



