THE PREPARATION OF AGAR AND GELATIN It 



Place the dish containing the mixed agar and bouillon in a 

 closed water bath and boil for 20 minutes. Then cool it to 

 a temperature between 45 and 50° C. and add the white of 

 one egg and thoroughly mix it in the liquid agar. This is 

 easily accomplished by pouring it a number of times from 

 ope beaker to another. When the egg albumen is dissolved, 

 the agar is returned to the water bath and boiled vigorously 

 until the white of the egg is coagulated. .This usually takes 

 about 20 minutes. Filter the agar itnmediately, while hot, 

 through ordinary filter paper which has been moistened with 

 boiling water. Distribute the filtrate in small and large tubes, 

 as directed. Sterihze, label, and store the agar in the same 

 manner as the gelatin. 



