PREPARATION OF CULTURE MEDIA 6/ 



(S) Small test tubes of bouillon containing i% grape sugar 



(dextrose, glucose). 

 (S) Small test tubes of bouillon containing i% milk sugar 



(lactose) . 

 (S) Small test tubes of bouillon containing \<Jo cane sugar 



(saccharose) . 



Group C. — (Media favorable for determining the produc- 

 tion of gas.) 



(S) Small test tubes of agar containing i% grape, sugar. 

 (5) Small test tubes of agar containing 1% milk sugar. 

 (5) Small test tubes of agar containing 1% cane sugar. 



Group J). — (Media and tubes favorable for approximate 

 gas analysis and the determining of the aerobic or anaerobic 

 tendencies.) 



(4) Fermentation tubes containing i % grape sugar. 

 (4) Fermentation tubes containing i % milk sugar. 

 (4) Fermentation tubes containing i % cane sugar. 



(The fermentation tubes containing bouillon with sugars may 

 be substituted for the media containing sugars in Groups B and 

 C if desired.) 



Group -E. — (Media either necessary for, or especially desir- 

 able for, the cultivation or differentiation of certain pathogenic 

 bacteria.) 



Acid agar (put in small test tubes) . 



Acid glycerin agar (put in small test tubes) . 



Acid glycerin bouillon (put in small test tubes). 



Blood serum (dog) solidified at 70-75° C. (ground-glass-capped 



tubes) . 

 Loeffler's blood serum (usually small test tubes) . 



103. The Preparation of Acid Agar. — This is prepared the 

 same as ordinary agar (17) with the omission of the sodium 

 hydrate in the bouillon from which it is made. 



