BACTERIUM SEPTIC^MIyE HEMORRHAGICA 8 5 



EXERCISE XL 



BACTERIUM (BACILLUS) SEPTICEMIA HEMORRHAGICA 

 AND MICROCOCCUS LANCEOLATUS {Continued) 



131. Work for this Exercise. — Reexamine all the cultures 

 of these bacteria, paying special attention to the reactions of 

 the liquid cultures. 



Make the indol test with the cultures in sugar-free bouillon. 



Make, stain, and examine a cover-glass preparation from an 

 organ or the blood of a rabbit which has died from the inocu- 

 lation with swine-plague bacteria, and also a preparation made 

 from a rabbit which has died from the inoculation with Micro- 

 coccus lanceolatus. Stain the preparations with an aqueous 

 solution of fuchsin. Study the bacteria in these preparations 

 and carefully compare the two. Indicate in the notes the 

 differences, if any are found. 



Give in the notes a list of characters and cultural properties 

 of Bad. sepiiccBfnitB hemorrhagica (swine plague) that are of 

 differential value. In what properties does it differ from B. 

 cholera suis ? 



Keep the cultures until the next exercise and compare them 

 again, after which they may be rejected. 



