BACTERIUM MALLEI 89 



EXERCISE XLII 



BACTERIUM (BACILLUS) MALLEI 



136. This organism grows most characteristically on potato 

 and somewhat feebly in the other media heretofore used. It 

 develops readily on acid agar and in acid glycerin agar and 

 acid glycerin bouillon. For this reason it is not inoculated 

 into all of the media. In diagnosing glanders, it is customary 

 to inoculate guinea pigs with the suspected material {see 

 Appendix III). From the lesions in these animals, if the 

 disease develops, pure cultures can usually be obtained. It 

 can be identified by its morphologic and cultural characters. 



References. — Chapters on Bacterium mallei in text books. 

 Smith, The Jour, of Conip. Med. and Vet. Archives, March, 1890. 

 deSchweinitz and Dorset, Jour, of the Am. Chem. Soc, Vol. 

 XVII. (1898). Finkelstein, Centralb. f. Bakteriologie u. Parasi- 

 tenkunde, Bd. XI. (1892), S. 433. 



137. Work for this Exercise. — Inoculate a tube of potato, 

 one of agar, one of acid agar, one of acid glycerin agar, one 

 of glucose agar, and one of bouillon from a culture furnished. 

 (The special media here introduced will be furnished by the 

 instructor.) 



Stain cover-glass preparations (furnished) made from the 

 lesions in guinea pigs which were inoculated with this organ- ' 

 ism. Stain one with alkaline methylene-blue and one with car- 

 bol fuchsin. Note especially the morphology and the extent the 

 organisms take the stain. 



