90 LABORATORY BACTERIOLOGY 



EXERCISE XLIII 



BACTERIUM (BACILLUS) MALLEI {Continued) 



138. Work for this Exercise Examine and carefully de- 

 scribe all the cultures of Bacterium mallei. 



Make 3 cover-glass preparations from the acid agar and 

 from the bouillon cultures, and stain one of each with alkaline 

 raethylene-blue, one with carbol fuchsin, and one with an aque- 

 ous solution of methyl-violet. Describe the bacteria and make 

 a drawing of a few of them. Preserve i preparation. Keep 

 the cultures and reexamine them at each of the 3 follow- 

 ing exercises. Note especially the character and color of the 

 growth on the potato. 



Examine carefully the lesions produced by the inoculation 

 of Bacterium mallei in a male guinea pig. 



Inoculate a tube of liquid agar (Liborius' method, paragraph 

 89) and a fermentation tube of glucose bouillon from a cul- 

 ture of tetanus bacilli (furnished) and place the inoculated 

 tubes in the incubator. 



