I02 LABORATORY BACTERIOLOGY 



pieces of the tissue in alcohol or in some other fixing fluid for 

 sectioning and staining, preparatory to studying them.) 



Preserve one or more of the cover-glass preparations to 

 accompany the notes. 



155. Making Cultures from Animal Tissues — Heat a plati- 

 num spatula to a red heat in a gas flame and scorch the surface 

 of the organ. Flame a pair of fine forceps and tear an open- 

 ing through the scorched surface and crush a bit of tissue 

 underneath it. With the platinum loop take up a loopful of 

 the crushed tissue with which inoculate the media. . It is also 

 desirable" to inoculate a tube of slant agar with the needle by 

 drawing it over the surface of the medium after charging it 

 with tissue. In making plate cultures use a loopful of the 

 crushed tissue for the first tube. The quantity of the tissue 

 necessary to give a desired number of colonies cannot be an- 

 ticipated, although experience in working with different organ- 

 isms in animals renders one able to approximate the amount 

 required. 



