Germination of Teleutospores of Ustilaginee. 83 
along to its growing end. Frequently two promycelial 
spores become united by a transverse bridge (conjugation) ; 
one of them then gives off a germ-tube, as in the case of 
single promycelial spores, only it is longer. 
U. grandis——In 1876, Kiihn* investigated the germina- 
tion of this species. He found that the promycelia, which 
are about from 50 to 6ou long, and from 5 to 8 wide, had 
a great tendency to fall off from the teleutospores before 
they produced promycelial spores, although this was not 
by any means always the case. Brefeldft found in nahrlé- 
sung that the promycelia were not only larger, but produced 
more and also larger promycelial spores. These sporidia 
not only reproduced themselves, but also grew into sporo- 
phores (Fruchitréger), which were indistinguishable from 
the ‘original promycelia, inasmuch as they were cylindrical 
septate tubes, which in their turn budded off spores. Thus 
colonies of yeast-cells do not occur in the life-cycle of 
U. grandis any more than they do in that of U. longéssima. 
With U. grandis the promycelium produces spores which 
grow out into sporophores, and they in their turn produce 
spores again. These sporophores are multicellular. 
U. bromivora, Tul.{—In water each spore produces a 
small promycelium through a minute opening in the 
epispore, very much after the manner of U. longissima. 
This bears terminally a spore which soon falls away. 
Between the fallen-off spores conjugations are frequently 
to be seen ; sometimes they become uniseptate and buckle- 
jointed. In nahrlésung the typical germination takes place 
in the production of single promycelial spores from a short 
promycelium. These spores increase in size and become 
uniseptate, or, as Brefeld terms them,§ bicellular sporo- 
* Kiihn in Rabenhorst ‘‘ Fungi Europei,” cent. xxiii. No. 2299, fig. 
+ Brefeld, Zoc. cit., pp. 116~123, t. ix. figs. 17-26. 
t Cf Kiihn, ‘‘ Vortrages iiber Getreidebrand.” 1874. 
§ Brefeld, Joc. cé#., pp. 123-129, t. x. figs. 2-8. 
