Germination of Teleutospores of Ustilaginea. 95 
the six spores generally only three germinate, and pro- 
duce at their distal extremities secondary spores similar in 
size and shape to themselves.* 
U. primulicola, Magnus.—Pirotta {| found that the fresh 
ripe spores germinated in water in about ten hours, by 
emitting a short cylindrical promycelium, which at its 
extremity gave off three or four branches that became 
spores, measuring from 9 to 18 in length, and from 4 
to gu in width. These, while still attached, produced 
secondary spores from their ends. The secondary spores 
germinated by the protrusion of a germ-tube (about 3u 
wide, and from 10 to 20 times as long as the spore), into 
which the protoplasm migrated. Lateral conjugation was 
occasionally observed. 
This species occurred in 1884, in Rev. C. Wolley Dod’s 
garden, on P. farinosa. In August of that year I received 
some specimens from Mr. Dod. The spores germinated 
readily in water, and emitted short promycelia, which bore 
a cluster of promycelial spores as figured by Pirotta. I 
found that no spore-formation took place unless the end of 
the promycelium grew in the air. If a spore germinated 
at the bottom of a drop of water, the promycelium grew 
upwards through the water until it reached the air. In 
these cases the lower part of the promycelium became 
emptied of its protoplasm and septate, just as one sees in 
Tilletia (Plate VII. Fig. 26, 27). The promycelial spores 
varied from 12 to 20u in length, and were 4 or 5m in width 
(Figs. 28, 29, 30). After keeping the promycelial spores 
in nahrlésung for two hundred and sixty-four hours, no 
further spore-formation was observed; but they became 
septate and nucleate (Fig. 29). 
Melanotenium—The mycelium is principally inter- 
* Prillieux, Zoc. cit, 
+ Pirotta, ‘*Nuovo Giornale Bot. Ital.,” vol. xii. (1881), pp. 235-239, t. vi. 
