72 APPENDIX I. 
turgid young tissue. After a few hours in what direction, with 
reference to the epidermis, have the sections become coiled, 
and why are they so limber? How does similar material 
respond to the effect of ordinary water? 
Treat some filaments of an alga or a fungus in the same way 
and with 1.p. and h.p. study the results. 
(6) Use a strong sugar solution (5 per cent or more) and 
note the effect on yeast plants, other fungi, and some alga. 
Explain the results. 
EXPERIMENT 10. TO SHOW FOOD DIGESTION. 
Of Starch. 
With Fehling’s solution, as in Experiment 5, test some starch 
to prove the absence of sugar. If no sugar is present, boil some 
of the starch in pure water and permit it to cool. To obtain a 
plant enzyme, crush some malt and let it soak in pure water 
for one or two hours and then filter. Test the filtrate with 
Fehling’s solution to prove the absence of sugar; add this 
to the boiled starch, and keep warm. After some time test 
with Fehling’s solution to show the presence of sugar. What 
caused it ? 
EXPERIMENT 11. TO SHOW FOOD DISTRIBUTION 
(Circulation). 
(a) The Path of the Upward Movement of Nutriment (Sap). 
Take some entire plant with a well-marked tap root, eg. a 
small parsnip, also a young leafy shoot that has had a narrow 
zone of the bark removed. Cut off the root tip and the lower 
end of the shoot and place each in a colored solution (Eosine 
in water or common red ink) for a number of hours. By 
sections of the root and stem and the examination of the 
leaves, observe the path of the colored fluid. 
A tree may be girdled (i.e. a ring of bark removed) in the 
spring and it will produce leaves and live during the season 
Why? It will die the next fall or winter. Why? 
