Ill \'ETi:RIX.\m' PATHOLOGY. 



over crystals of iodine. The jjlycog-en appears as brownish areas 

 in the cells. It may also be demonstrated by immersing in a 

 mixture of fnur parts of alcohol and one part of tincture of iodine, 

 sections that have been hardened in absolute alcohol, the g"Iyco- 

 gen assuming a port wine color. 



Pathologic glycogenic infiltration occurs in pus cells of suppu- 

 rating processes. The blood cells, especially leucocytes, contain 

 some gl_\'COg"en in those animals affected with septic infection or 

 sapremia. Thus horses afflicted with sapremia induced b}^ punct- 

 ure wounds of the foot show glycogenic infiltration, of the blood 

 cells. In diabetes mellitus the liver and kidney cells are infil- 

 trated with excessi\'e cpiantities of gl}-cogen. 



Etiology. — Disturbed carbohydrate metabolism is insepar- 

 ably associated with glycogenic infiltration although the specific 

 relation of disturbed carbohydrate metabolism and glycogenic 

 infiltration is not known. Glycogenic infiltration has some asso- 

 ciation also \\itli certain infectious and inllamniatorv disturban- 

 ces as well as lun:or formations. 



Appearance. — Macroscopic. — (ilycogenic infiltration does not 

 produce lesions sulffciently characteristic to be recognized with- 

 out the aid of a microsco])e. 



Microsco[^ic. — The affected cells contain transparent colorless 

 areas near the nucleus. If the specimen has been hardened in 

 absolute alcohol, the areas of glycogen may be stained port 

 wine color by four ]3arts of alcohol and one part tincture of 

 iodine. The areas are \ariable in size depending upon the ex- 

 tent of the condition. In extreme cases the glycogen may be 

 observed in the intercellular spaces. 



Tissues Affected. — Li\-er, kidney, muscle, and blood cells are 

 most subject to glycogenic infiltration, the frequency in the order 

 named. 



Effects. — The glycogen is readily reabsorbed provided the 

 cause be removed. The condition being associated with other 

 pathologic conditions, their removal becomes essential before 

 the glycogenic infiltration can be overcome. 



