44 BULLETIN 431, U. S. DEPAETMENT OF AGBICULTtTBE. 



Table XI. — Resistance of saebrood virus to putrefaction. 



Date of inoculation. 



Time of 

 putrefac- 

 tion. 



Results of inoculation. 



Aug. 6, 1914., 

 Aug. 7, 1914. 

 Aug. 10,1914 

 July 20, 1915. 

 Sept. 13, 1916 

 Sept. 14, 1915, 

 July 22, 1915. 

 July 8, 1915.. 

 May 22, 1915. 

 Sept. 22, 1915, 

 Aug. 18, 1915. 

 Sept. 16, 1914. 

 Sept. 25, 1914, 

 July 1,1915.. 



Days. 

 1 

 2 

 3 



Saebrood produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. " 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 



Do. 



From Table XI it will be noted that the virus of saebrood was 

 destroyed ia the experiments recorded in from 7 to 10 days. As in 

 the case of fermentation, so in the case of putrefaction, it is to be 

 expected that the time for the destruction of the virus will vary 

 appreciably with the temperature at which the putrefactive processes 

 take place. 



RESISTANCE OF SACBEOOD VIRUS TO CARBOLIC ACID. 



Larvse recently dead of saebrood are crushed and strained. This 

 larval mass is diluted with carbolic acid in aqueous solution. About 

 10 parts of carbohc acid to 1 part of the larval mass is used. This 

 suspension is distributed in test tubes and allowed to stand at room 

 temperature. Each tube contains the virus from, about 15 larvse. 

 After periods, reckoned in days, colonies free from disease are inocu- 

 lated, each with the contents of a single tube added to sirup. 



Carbolic acid solutions of J, 1, 2, and 4 per cent were used in mak- 

 ing the suspensions. The following results were obtained from the 

 experiments : 



Table XII. — Resistance of saebrood virus to carbolic acid. 



Date of inoculation. 



Kesults of inoculation. 



Sept. 3, 1914. 

 Sept. 18, 1914 

 Sept. 3, 1914 . 

 Sept. 17, 1914 

 Aug. 12, 1915, 

 Aug. 20, 1915, 

 May 14, 1915. 

 Sept. 3, 1914. 

 Sept. 18, 1914 

 June 23, 1915. 

 Sept. 17, 1915 

 Aug. 12, 1915. 

 Aug. 21, 1915. 

 June 4, 1916.. 



Saebrood produced. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 

 Saebrood produced. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 



