15 

 INTRODUCTORY AND TECHNIC. 



LABORATORY WORK FOR THE WEEK. 



I. Imbedding tissue. Go to the delivery desk with two 

 small shell vials filled with 82% alcohol from the supply shelf and 

 get two pieces of tissue already fixed and hardened and now in 

 82% alcohol. One of these is to be imbedded according to the par- 

 afiBne method and sectioned during the week of the 

 term The other piece is to be stained in toto in para- 

 carmine, imbedded in collodion, and sectioned during the 



week of the term. By means of a ribbon pin attach to the cork of 

 each bottle a label giving the name of the animal, the tissue or 

 organ, the fixer employed, the method in which it is to be imbedded, 

 and the date. 



In order that jou accomplish this successfully it is necessary 

 for you to study carefully §§ 39-47 ; 50-60. Keep a record of the 

 steps and the times of transfer to each fluid and present it as part 

 of the laboratory report on due . (See 



bulletin board). 



II. Fixation of tissue. Fill two medium shell vials with 

 picric- alcohol and go to the delivery desk for two pieces oi fresh 

 tissue, to be 7?j:(?fl? and hardened, and imbedded according to (a) the 

 parafl&ne method, and {h) the collodion method. Your name, to- 

 gether with the tissue you are to receive and the imbedding method 

 to be employed is posted on the bulletin board. 



Label the specimen, giving the name of the animal, the organ 

 or part, the fixation method, imbedding method, and the date. 



Keep a record of the steps in the process, and submit it as part 

 of the report for the week of the term. 



In order that this work be successfully pursued, it is necessary 

 to study carefully §§ 18-21 ; 25 ; 39-47 ; 50-60. Be sure that you 

 understand the order of succession of the various steps and the rea- 

 son for them, and follow directions absolutely. Fix in picric-alcohol 

 1-2 days, place in 67% alcohol 1-2 days, in 82% alcohol several days, 

 changing the alcohol to fresh three or four times (§ 25). In 

 this work, be as independent of assistance from the instructors as 

 possible. 



