i6 



III. Isolation. From the stock bottle on the shelves, fill a 

 small shell vial with Miiller's fluid dissociator and go to the delivery 

 desk for a small piece of trachea of cat (sheep or calf). Label it, 

 naming the animal, tissue, and dissociator. Leave the tissue in the 

 dissociator until you come at the second period (1-2 days) when the 

 action of the dissociator will have been sufiicient. For this work 

 study carefully §§ 2-7. 



At your second period, in accordance with the directions in 

 §§ 5"7> (^) examine the isolated cells unstained, in dissociator ; (6) 

 stain a second preparation with methylgreen and eosin (§92, b), 

 and mount in glycerin ; {c) mount a third preparation of the cili- 

 ated cells in alum-carmine and eosin glycerin (§ 156). 



IV. Label the bottles of clearer ; fill and label the bottle for 

 distilled water and those for 95% alcohol and normal salt solution ; 

 prepare and label in their respective bottles 1 10 c. c. of picro- 

 fuchsin and 90 c. c. of 67% alcohol. 



V. Make 4 shellac rings (cells) for % in. cover glasses ; pre- 

 pare two for ]4, in. covers (§ 106). 



VI. Read carefully §§ i ; 18-21 ; 64 ; 71-78. 



Review what you have so far done on I, II, and III. 

 Study carefully §§ 64-70 ; 79 ; 1 00-112. 



THE CELL AND THE TISSUES. 



References : 



Points for Quiz : 



Laboratory Report : 



Due : 



I. Ovarian Ova. Starfish {Asterias Forbesii). Assigned 

 for study. Picro-acetic fixation ; paraffine ; hematoxylin and 

 eosin. Study, noting the nucleus (germinal vesicle), cell-body 

 (vitellus), nucleolus, nuclear membrane, and structure of the 

 nucleus and cell- body (spongioplasm and hyaloplasm). This prep- 



