26 



CONNECTIVE AND SUPPORTING TISSUE. 



LABORATORY WORK FOR THE WEEK. 



References : 

 Points for Quiz : 

 Laboratory Report : 



Due : 



Go to the delivery desk (a) for 35, assigned for study ; (^) with 

 a slide prepared for a paraffin section, 32 ; {c) with a clean slide for 

 collodion sections, 37, 38, 39 ; fasten to the slide according to § 66 ; 

 place in benzin. 



While the above preparations are being carried on (§§ 68-70) go 

 to the desk for 33 and 34, from which free-hand sections of the car- 

 tilage are to be cut, stained and mounted as directed below. Return 

 the tissue to the desk when you have finished with it. 



During the week you will make preparation 36, in accordance 

 with the directions, §§ 133-134. Read them carefully in advance. 



CARTILAGE. 



32. Embryonal cartilage. . Transection of 



; ; stained in toto (§ 74, c, § 86) in 



paracarmine ; paraffin ; sections fx. Mount in balsam (benzin, 



clearer, balsam). 



The skull should be studied. It is at this stage cartilaginous, 

 and the cartilage in an embryonic stage ; note the cells with their 

 nuclei and cell-bodies ; the small amount of matrix deposited be- 

 tween them, and compare with adult cartilage in 33 and 38. What 

 would be the structure of the cartilage at an earlier stage of develop- 

 ment ? 



33. Hyalin cartilage. Nedurus (Amphibian). You will 

 receive one of the following from which to cut free-hand sections of 

 cartilage, (a) head of the humerus ; (6) head of the femur ; (<:) a 

 branchial arch ; (a?) part of the hyoid arch ; (e-) the pectoral arch ; 

 (/) the pelvic arch. 



