29 



osteum. Study carefully the centers of ossification in the epiphysis 

 and shaft, so as to understand the dissolution of the cartilage (de- 

 generation of the cells, absorption of the matrix) and the deposit of 

 bone. How is spongy bone converted into dense bone ? Study the 

 structure of the periosteum, recognizing the two layers ; understand 

 the part it plays in the processes of ossification. In addition, .study 

 the preparation for the structure of cartilage, articular cartilage, the 

 general structure and articulation of a long bone. 



39. Transection of a long bone. ; Picric 



alcohol ; decalcified in nitric acid decalcifier (§ 131) ; collodion ; 

 sections fx. Stain in hematoxylin minutes, eosin 



seconds. Mount in balsam . 



Study, noting (a) the marrow with its nutrient artery, {b) the 

 structure of the bone, (c) the structure of the periosteum. Com- 

 pare the structure of the decalcified bone with the dry preparation 

 of bone, 36. Recognize the identity of structure in the two prepar- 

 ations so differently prepared. 



