34 

 BLOOD AND LYMPH. 



LABORATORY WORK FOR THE WEEK. 



References : 



Points for Quiz : 



Laboratory Report : 



Due : 



Go to the delivery desk for 51; stain as directed below and 

 mount in balsam. Next prepare 53. Make the remaining prepara- 

 tions in order. 



LEUCOCYTES. 



49. Amoeboid movement. Amphibian leucocytes. Place 

 upon the slide a drop of frog's blood ; apply a cover glass at once 

 and seal the preparation with castor oil. 



Search the preparation until a leucocyte is found undergoing 

 amoeboid movement. Keep it under observation for some time, ob- 

 serving the changes of form and mode of progression. Make sev- 

 eral sketches at intervals of 2-3 minutes showing the changes. 



50. Amoeboid movement. Human leucocytes. Place a 

 drop of fresh human blood upon a slide, cover quickly and seal with 

 castor oil. Ten or fifteen minutes after the preparation was made 

 examine it, find a leucocyte in amoeboid movement. Study it care- 

 fully and make several sketches at intervals, as in 49. 



51. Ingestion of foreign particles. Leucocytes of rat. A 

 solution containing particles of lampblack (§ 157) was injected 

 into the abdominal cavity of a rat ; thp following day the rat was 

 killed and smears were made of the abdominal lymph. Stain for 

 ID minutes with hematoxylin (§ 124). Mount in balsam. 



Study the preparation, noting that the particles of lampblack 

 have been taken up or ingested by the leucocytes of the lymph. 

 Consider, in the light of 49 and 50, how this ingestion took place. 



(43) Forms of leucocytes. Study the preparations 43, (a) 

 and (b), searching carefully for different kinds of leucocytes. The 

 following types will probably be found : (a) small lymphocytes, 



