114 



Paraffin or Collodion Sections 

 (§§66 and 67) 



V 



Benzin 



95 % Alcohol 



V 



Water 



V 



Hematoxylin, 10-30 min. 



V 



Water 



V 



Picrofuchsin. eosin 

 or erj'throsin, 1^-2 min. 



V 



Distilled water or 67 % alcohol 



V 



Dehydrate (95-99 % alcohol) 



V 



Clear 



(carbol-xylene) 



V 



Mount in Balsam 



STAINS. 



I 80. Chloral Hematoxylin. (Proc. Am. Micr. Soc, vol. XIV, pp. 125- 

 127). formula: (A) Hematoxylin crystals, y\; gram, dissolved in 10 c.c. of 

 95% alcohol ; (B) distilled water, 100 c.c, potash alum, 3^^ grams ; boil for 5 

 minutes in an agate-ware or glass vessel and add suflScient boiled water to bring 

 the volume back to 100 c.c. When cool, mix (A) and (B) and add chloral hy- 

 drate, 2 grams. Allow the solution to ripen for a week or two in the air and 

 sunlight, or add 1-2 c.c. hydrogen dioxid, when it is ready for use. Its staining 

 quality improves up to an optimum and then begins to deteriorate. Old 

 hematoxylin generally contains a precipitate and should be filtered often or 

 before using. 



Stain sections from water for 5 .30 minutes according to the age of the solu- 

 tion, the character of the tissue and the fixation employed. After staining, 



