"5 



wash well with tap water to give a blue tone to the stain. Counter-stain as 

 desired. 



S 8i. Ehrlich's acid hematoxylin. Formula: Water, looc.c. ; 95% alco- 

 hol, ibo c.c. ; glycerin, 100 c.c. ; glacial acetic acid, 10 c.c. ; alum in excess. 

 Let the mixture ripen in the light until a dark red. Sections stain in this hem- 

 atoxylin in a short time, generally 5-10 minutes. Wash with water after 

 staining. 



\ 82. Delafield's hematoxylin. Formula: Saturated aqueous solution of 

 ammonia alum, 200 c.c. ; 16% alcoholic solution of hematoxylin cr3'stals, 11% 

 c.c, {hematoxylin crystals 2 grams, 95% alcohol I2j^ c.c, ). Allow the mix- 

 ture to stand in the light and air in an unstoppered bottle for 4 or 5 days ; filter 

 and add glycerin, 50 c c, and methylic alcohol, 50 c.c. Permit it to stand for 

 a week or so to ripen ; filter and keep in a stoppered bottle. The staining power 

 increases for several months. In using, dilute 3 or 4 times with distilled water. 



Stain sections from water ; 4-5 minutes will generally be sufiicient. Wash 

 well with water after staining. 



This is a very strong hematoxylin stain and may be used to advantage with 

 tissues that stain with difficulty. It is likewise a more diffuse stain than either 

 chloral or Ehrlich's hematoxylin, staining cell-body as well as nucleus, — a fea- 

 ture having its advantages and disadvantages. Old solutions (several months 

 to a year) should be filtered before using. 



\ 83. Weigert's hematoxylin. Formula : Hematoxylin, i gram, dis- 

 solved in 95% alcohol, 10 c.c. ; distilled water, 90 c.c. ; saturated aqueous solu- 

 tion of lithium carbonate, i c.c. This stain may be used at once or (better) be 

 allowed to ripen for 2-3 days. 



Tissue should be stained from water and rinsed in water after staining. 

 This is a powerful stain and is used in Weigert's method of staining myelinic 

 nerve fibers ( ? 1 36 ) . 



§84. Borax carmine. (Grenacher), Formula: Borax, 4 grams; car- 

 mine, 3 grams ; water, 100 c.c. ; allow the mixture to stand for several days, 

 shaking occasionally when most of the carmine will have dissolved ; filter and 

 add 100 c.c. of 70% alcohol. Let the mixture remain for several days, filter 

 again again and the solution is ready for use. 



This is a good carmine stain for in ioto staining. Stain objects in toto for 

 one to several days, according to size; remove to 67% (lo%) alcohol, acidu- 

 lated slightly with hydrochloric acid, (4 drops in each 100 c.c), for a day and 

 then remove to 82% alcohol. It affords a bright red stain that is quite trans- 

 parent. 



g 85. Alum carmine. (Grenacher). Formula: Carmine, i grm ; potash 

 (or ammonia) alum, 4 grams { 1-5 grams) ; water, 100 c.c. ; boil for 15-20 min- 

 utes, allow it to cool and filter. 



A good carmine stain for sections. Stain 5-30 minutes, or longer if neces- 

 sary ; rinse off the excess of stain with distilled water. The stain is a purple- 

 red. 



