119 

 Iron Hematoxylin 



Any Fixation desired 



V 



Paraffin Sections 



V 



Benzin 



V 



95 % Alcohol 



V 



Water 



V 



Mordant 

 1-3 hours in 4 % ferric alum 



Water 



V 



Stain 1-6 hours 

 in ^ % aq. hematoxylin 



V 



Water 



V 



Differentiate, by dipping alternately in 



mordant and in the tap-water, 



a second in each 



I 



V 



Water (running) 

 15 minutes 

 I or 



V 



Orange G. 

 (;^ sat. aq. sol.) 



V 



Water (rinsed) 



— > 95-99 % Alcohol 



I 



V 



Clear in Carbol-xylene 



V 



Mount in Balsam 



