§ 99. Staining with Safranin 



Flemming's or Hermann's 

 fluid fixation (best) 



V 



Paraffin sections 



V 



Benzin 



I 



V 



95 % Alcohol 



I 



V 



Safranin (Babes'), 

 2-12 hours 



I 



V 



Distilled water 

 (rinsed) 



I 



V 



95 % Alcohol 



I 



Acid 95 % alcohol (tV Hcl.) 

 for a few seconds 



V 



95-99% Alcohol 



I 



V 



Carbol- xylene 

 I 



V 



Mount in Balsam 



MOUNTING. 



§ 100. Whether stained or unstained, prepared for microscop- 

 ical examination by isolation or sectioning, and especially if it is de- 

 sired to keep the preparation, it is necessary to moutit it in some 

 way, — i. e., so arrange it upon some suitable support (glass slide) 

 and in some suitable mounting medium that it may be satisfactorily 

 studied with the microscope. 

 Mounting may be 



I. Temporary, or 

 II. Permanent, — as 



A. Dry, or in air, 



B. In a medium miscible with water, or 



C. In a resinous medium, in which case it is neces- 



