30 LABORATORY GUIDE IN BACTERIOLOGY 



The weight of the mixture should be i,ooo grams. 

 Tube hot, without filtration, through a pipette with a 

 wide aperture. Autoclave for five minutes at 120° C, 

 and allow to cool in a slanting position. 



EXERCISE 7. STANDARD METHOD OF PREPARING BROTH, 



NUTRIENT GELATIN, AND NUTRIENT AGAR 



STANDARD METHODS FOR TBE EXAMINATION OP WATER AND 



SEWAGE. AMERICAN PUBLIC HEALTH ASSOCIATION, 



7SS BOYLSTON STREET, BOSTON, MASS. 



g. Titrate after boiling one minute to expel carbon dioxid. 

 10. Adjust reaction to 1% acid by adding normal hydrochloric add or sodium 



hydrate as required, 

 zi. Heat over boiling water (or steam) bath for 40 minutes. 

 12. Restore loss by evaporation. 



