34 LABORATORY GUIDE IN BACTERIOLOGY 



Drigalski and Conradi's medium (modified by 

 Harris) — 



Dextrose-free broth 2,000 c.c. 



Nutrose 20 grams 



Agar 40 grams 



Boil, dissolve, neutralize to phenolphthalein, auto- 

 clave at 120° C. for 5 minutes. Clarify with whites of 

 four eggs (or powdered egg albumin), and filter. Then 

 add after dissolving separately in water: 



Lactose 30 grams 



Litmus solution (see p. 35^ 260 c.c. 



Crystal violet (o.i per cent solution) 20 c.c. 



Tube and sterilize in the arnold. 



Parietti's solution — 



Carbolic acid 5 c.c. 



Hydrochloric acid 4 c.c. 



Water 100 c.c. 



Phenol media. — One part carbolic acid (phenol) is 

 added to 1,000 parts medium. 



Hiss's plating medium for colon-tjrphoid differ- 

 entiation — 



Agar 15 grams 



Gelatin 13 grams 



Extract of meat , 5 grams 



NaCl S grams 



Dextrose 10 grams 



Distilled water 1,000 c.c. 



Hiss's tube medium — 



Agar 5 grams 



Gelatin 80 grams 



Extract of meat 5 grams 



NaCl S grams 



Dextrose 10 grams 



Distilled water 1,000 c.c. 



