BACTERIOLOGICAL TECHNIC 39 



Solution for testing the formation of nitrates — 



MgS04 0.3 gram 



FeSO^ 0.4 gram 



NaCl 0.5 gram 



K3HPO4 \. o.s gram 



Fused NazCOj i gram 



NaNQj.. I gram 



Distilled water 1,000 ex. 



Dissolve 20 grams agar in the above solution. 

 Sterilize in the autoclave. The salt solution may also 

 be added to the silica jelly. For plating fill in culture 

 tubes. 



Solution for testing the assimilation of atmospheric 

 nitrogen — 



I. Solution 



KH2PO4 I gram 



MgSO^ 0.50 gram 



NaCl o.oi gram 



FeS04 0.01 gram 



MnS04 0.01 gram 



Dextrose 20 grams 



Distilled water 1,000 c.c. 



Dissolve 20 grams agar in this solution if intended 

 for plating and tubing. Sterilize in the autoclave. 



2. Mannit Solution 



K2HPO4 o . 20 gram 



CaClj 0.02 gram 



MgS04 o. 20 gram 



FeaCk 0.01 gram 



Mannit 15 grams 



NaOH solution until neutral to phenolphthalem 

 Distilled water 1,000 c.c. 



Dissolve 20 grams agar in this solution if intended 

 for plating and tubing. Sterilize in the autoclave. 



