TABLE OF CONTENTS 



Part I. Bactekeological Technic 



Section 

 Section 

 Section 

 Section 

 Section 

 Section 

 Section 

 Section 

 Section 

 Section lo. 



Laboratory Rules 3 



General Directions 4 



Preparation and Cleaning of Glassware . 8 



Methods of Sterilization 10 



Preparation of Culture Media ... 18 



Preparation of Staining Solutions . ^ . 43 



The Microscope 45 



Scheme for Routine Study of Bacteria . 50 



Method of Describing Cultures ... 53 



Directions for Filling out Culture Charts ^8 



Part II. General Bacteriology 



Section 

 Section 



Section 

 Section 



Section 

 Section 



Section 

 Section 

 Section 9, 



69 



Preparation of Culture Media 

 Collecting and Cultivating Micro-organ- 

 isms from the Air 70 



Study of Molds, Yeasts, and Torulae . 78 

 Bacteriological Examination of Water, 



Air, and Milk ........ 84 



Exercises on Infection and Sterilization . 91 

 Influence of Disinfectants, Light, and 



Heat on the Growth of Micro-organisms 94 



Study of Chromogenic Bacteria ... 97 



Study of Micrococci 100 



Study of Intestinal Bacteria .... loi 



Part III. Important Pathogenic Bacteria 



Section i. Preparation of Culture Media ... 105 



Section z. The Pyogenic Group 105 



Section 3. The Group of Colon-Typhoid Bacilli . 112 



Section 4. The Proteus Group 121 



Section 5. The Capsidated Group 123 



Section 6. The Diphtheria Group 124 



