FROST'S CULTURE CHART (MODIFIED) 



Group 



Name of organism'^ 



Source 



HabiUt 



References 



Morphological Characters 



< 



•sa 



Sketches 



. Form: 

 a. On agar. 



Rod: 



b. On other media. 



Coccos: 



Spirillum: 



2. Size 



3. Cell groupings. 



4. Staining powers 



a. Anilia gentian violet , 



b. LSffler's methylene blue 



c. Gram stain 



d. Special stains, such as: (i) Flagella. . . (2) Spore . 

 {3) "Tubercle" (4) Capsule 



5. Motility: (a) Flagellar ... (4) Molecular 



c. Character of movement 



6. Spores 



7. Special characters, such as: 



capsules, vacuoles, granules 



pleomorphic and involution forms, etc 



Physiological Characters 

 Relation to temperature: (a) Optimum ...(&) Minimum . . . (c) Maximum . 



Relation to free oxygen: 



Relation to other agents such as: 



desiccation, light, disinfectants, etc: 



Figment production: 



Growth in carbohydrate media: 



a. Stab or shake culture (dextrose agar): gas production 



b. Fermentation tube: (i) growth in bulb:. .(2) growth in dosed arm:. 



c. Percentage of gas produced 

 ■ ' Dextrose 



Lactose 



Saccharose 



fermentation tube . 



After 24 hours 



After 48 hours 



Gas formula =q- in. . . 



d. Reaction (i) in bulb '. (2) in closed arm . 



Acid or alkali t)roduction (in litmus milk) 



Reduction of nitrates to.nitrites to ammonia 



Indol production; 24 hours 48 hours days 



fecal odor'; 24 hours 48 hours days 



Enzy m production; (o) proteolytic (i) coagulati ve (<r) diastatic . . 



Characteristic odor 



Agglutination 



Pathogenesis 



