SECTION I 



PREPARATION OF CULTURE MEDIA 



The following amounts of culture media will be 

 required in this work: 



The following staining solutions should be made up 

 in sufficient quantities to fill ordinary staining bottles: 

 Loffler's methylene blue. 

 Ziehl-Neelsen's carbol fuchsin. 

 Ehrlich's gentian violet. 

 Gram's iodin solution. 



The work in this course should begin with Part II, 

 Section 7, p. 97. 



SECTION 2 



THE PYOGENIC GROUP 



EXERCISE I. THE PYOGENIC GROUP (SUBGROUP a)' 



Members — 



Staphylococci, streptococci, Microc. tetragenus. 



Inoculate agar slants from laboratory cultures of 

 Staphylococcus aureus, Staph, albus, and Strepto- 



'This subdividing of the pyogenic group is an arbitrary 

 measure designed to facilitate study, the commoner pyogens 

 being studied first. 



