IMPORTANT PATHOGENIC BACTERIA 127 



SECTION 7 

 THE HEMORRHAGIC SEPTICEMIA GROUP 



Members — 



Bacillus pestis (bacillus of bubonic plague). 

 B. cunicuUcida (bacillus of fowl cholera, bacillus of rabbit 

 septicemia, Bacillus der Rinderseuche, Bacillus der 

 Schweineseuche, etc.). 

 Reference — 



Moore, The Pathology of Infectious Diseases of Animals. 



Inoculate agar slants from a laboratory culture of 

 B. cunicuUcida or another organism of this group. On 

 account of the enormous infectiousness of B. pestis it is 

 not desirable to study this organism in classwork. 



1. Routine study. — Stain with Loffler's methylene 

 blue andanilingentian violet. Observe "polar staining." 



2. Special study. — Inoculation of a rabbit sub- 

 cutaneously or by scarification. When dead, study in 

 the usual manner, and observe the hemorrhages pro- 

 duced in the serous membranes. Make cultures from 

 the heart's blood, where large numbers of bacilli will 

 be found. Also make a stained preparation from the 

 heart's blood, and note the typical polar staining. 



SECTION 8 



THE ANTHRAX GROUP 

 Members — 



Bacillus anthracis. 

 B. subUlis. 



Great caution is necessary in handling B. anthracis. 

 Inoculate agar slants from laboratory cultures of 

 B. anthracis and B. subtilis. 



