134 LABORATORY GUIDE IN BACTERIOLOGY 



SECTION 12 

 PATHOGENIC TRICHOMYCETES 

 Members — 



Actinomyces bovis (Jiominis) and Actin. asteroides. 



Leptothrix. 



Cladothrix. 



Nocardia. 



1. Inoculate broth and potato from laboratory cul- 

 tures, and make descriptions and stained preparations 

 as usual. Other media need not be inoculated. 



2. Special study. — Suspend a small amount of the 

 potato culture in physiological salt solution, and 

 examine under the low power. 



3. Special study. — Examine a sample of actinomy- 

 cotic tissue (bovine) in the fresh state, for so-called 

 "sulphur granules." Crush some of the material in 

 salt solution under cover slips and search for "clubs," 

 using the low and high power dry lenses, and stain by 

 Gram's method; counterstain with eosin or Bismarck 

 brown. 



SECTION 13 



THE GROUP OF ANAEROBIC BACILLI 



Members — ■ 



Bacillus tetani. 

 B. edematis. 

 B. welchii. 

 B. chauvei, 

 B. botulinus. 

 And others. 



Anaerobic cultivation involves the growth in an 

 atmosphere devoid of oxygen. This is accomphshed 

 by removing the oxygen from air by chemicals, or 



