MOLDS, YEASTS, TORULAE, AND BACTERIA 193 



6. Incubate one flask at room temperature, one at 

 37° C, and one at 40" C. 



7. Examine and note the degree of film formation 

 at the different temperatures and the differences in 

 morphology. 



8. Add small amounts of alcohol from time to time. 



9. Titrate daily with 1/20 N • NaOH and determine 

 the amount of acid produced. 



EXERCISE 2 



Inoculate liquid wort to which 3 per cent saccharose 

 has been added with an alcohol-forming yeast. After 

 alcohol has been produced, inoculate with one of the 

 acetic-acid cultures and note the amount of acid 

 produced. 



