208 LABORATORY GUIDE IN BACTERIOLOGY 



Holler's grass bacillus, 131. 



Mailer's spore stain, 129. 



Moist chamber, Bottcher's, 183. 



Mold spores, gennination of, 83. 



Molds, 182; amylolytic action of, 

 184; in cheese, 184; in milk, 

 160; microscopic examination 

 of, 184; yeasts, torulae, and 

 acetic-acid bacteria, 179. 



Molecular movement, 75. 



Mouse: holder, in; microscopic 

 examination of, in. 



Muscle sugar, loi. 



Must, wine, 41. 



Natural souring of milk, 156. 



Needles, platinunj, 6. 



Negative Gram stain, 78. 



Neutral-red agar, 33. 



NicoU^'s carbolic gentian violet, 

 45; carbolic thionin blue, 44. 



Nitrate: bacteria, 174; broth, 

 41; formation, 174; media, 41; 

 solution, 41. 



Nitrates, test for, 175. 



Nitrite: bacteria, 171, 172; for- 

 mation, 171. 



Nitrites, test for, 114. 



Nitrogen, assimilation of, 175. 



Nitroso-indol reaction, 130. 



Nocardia, 134. 



Non-protein media, 4s. 



Normal solution, 20. 



Novyjar, 137. 



Ox-bile medium, 35. 



Ox-blood serum, 33. 



Parietti's solution, 34. 



Park's method of anaerobic culti- 

 vation, 135. 



Pasteurizing milk, 89, is8. 



Pedesis, 76. 



Pepton: broth, iSj gelatin, 26; 

 solution, Dunham's, 18, solu- 

 tion for ammonification, 40. 



Peptonization, 62, 168. 



Petri dishes, 7. 



Phenol: media, 34; sulphonic acid, 

 I7S- 



Phenolphthalein, 19. 



Phenomena: of infection, 91; of 

 sterilization, 91. 



Pigments, 100. 



Plate cultures, 98. 



Plating medium. Hiss's, 34. 



Plugging culture tubes, 9. 



Pneumobacillus, 123. 



Pocket inoculation, 125. 



Polar staining, 116, 127. 



Positive Gram stain, 78. 



Potato, 28; glycerinated, 37; 

 substitute for, 29; tube, 28. 



Preparation: Gram, 77; hang- 

 ing drop, 75 ; impression, 128; 

 of agar-agar, 20; of blood 

 agar, 40; of blood serum, 31; 

 of bouillon, 18; of broth, 18, 

 31; of culture media, 18; of 

 dextrose agar, 26; of Dunham's 

 pepton solution, 18; of egg 

 media, 37; of gelatin, 26; of 

 glycerin media, 36; of litmus 

 milk, 27; of media for soil ex- 

 amination, 37; of media for 

 water and milk examination, 35; 

 of non-protein media, 42; Pres- 

 cott and Breed's method, 161; 

 of silica jelly, 38; of soil samples, 

 166; of soil suspensions, 166; of 

 staining solutions, 43; of sugar- 

 free broth, loi; erf whey media, 

 36; stained, 76. 



Proteolysis, 62. 



Proteolytic enzym, 62. 



Proteus: group, 121; vulgaris, 

 121; zenkeri, 121. 



Pure culture: of bacteria, 187; 

 of yeasts, 187. 



Pus, gonorrheal, 109. 



Pyocyanin, 100. 



Pyogenic group, 105. 



Rabbit septicemia, 127. 

 Rain water, 148. 



