BETTS ¢ THE FUNGI OF THE BEE-HIVE. 149 
that of C. glaber. This species does not appear to produce any 
colouring matter, nor to discolour the medium on which it grows. 
In sulphuric acid all parts of the fungus are rendered very 
transparent; on heat being applied, the conidia only remain. 
Hydrochloric acid produces no immediate effect; on heating, the 
conidia remain, also some hyphae; but the latter are considerably 
disorganised. Caustic potash extracts a yellow colouring matter 
from the conidia even when cold. 
This species is more tolerant of high temperatures than C. 
glaber. Cultures at 269-42° C. germinated, and in some cases pro- 
duced a few conidiophores. At room temperature the fungus did 
well, also under outdoor conditions (May), 
; 
ce 
2 
Para 
ee ee 
Fig. 16.—Citromyces subtilis. Conidiophore and conidia. x 1,400. 
Only one gelatine culture was made; after 15 days some 
liquefaction had taken place. Several cultures on media with litmus 
were made; in no case was any, reddening of the medium seen, SO 
that acid is evidently not produced. The fungus is therefore pro- 
bably C. subtilis; Bainieér and Sartory (1, p. 46) describe this species 
as peculiar in that it produces no citric acid. Their specimens had 
conidia 2-2.5 p, inflated apex 8-10 »; in the manner of branching 
they resembled the fungus here described. Bainier and Sartory’s 
species liquefied gelatine slowly; liquefaction began 16,or 17 days 
after germination. 
