148 BETTS | THE FUNGI OF THE BEE-HIVE, 
tawny-yellow in colour, 680-500 x 560-400 /, and WEES Bae a 
embedded in the mycelium. They were evidently immature when 
examined; asci were not found. 
7 ae a es colour of the vegetation is similar to that of Pent- 
cillium, but lighter and greyer. The fungus produces a yellow or 
brown coloration on some media (prune decoction agar and honey 
gelatine, for example); when grown on rice, it colours the rice a 
bright yellow (see Wehmer, 28). ; 
The conidia and hyphae survive immersion in cold sulphuric 
acid; on the application of heat, the conidia only remain. A similar 
result ensues on heating in hydrochloric acid. Cold hydrochloric 
acid and caustic potash solution do not produce any immediate effect 
on the specimens. Hot caustic potash solution extracts a yellow 
colouring matter from the conidia. 
This species is unable to endure high temperatures; the cultures 
at 26°-42° C. were all killed. Germination took place out of doors 
in May as quickly as at room temperature (in three days), but was 
inhibited during the winter. At room temperature the fungus did 
well. These observations are in accordance with Wehmer’s results 
(28); he gives the limits of germination as 8°-32° C., optimum 
209-259 C. The swollen apex of the conidiophore, in his specimens, 
measured 4-15 p, the sterigmata 9-12 x 3-4 mw. In other respects the 
fungus here described agrees well with Wehmer’s. 
This species liquefies gelatine; a considerable quantity of acid 
is produced, as is shown by the reddening of media containing 
litmus. The vegetation was grey, instead of green, on bouillon 
agar. The fungus grew well on a 5 per cent. solution of citric acid 
to which 2 grm. of agar per. 100 ccm. had been added. (The 
medium remained liquid). 
Citromyces subtilis, Bainier and Sartory. 
This species is very common in hives, whether more so than the 
last it is not possible to say, as they resemble each other closely, and 
have doubtless often been confused. 
The conidiophore is 3 » in diameter, generally branched, having 
a septum just above the branch. The apex is slightly inflated, 3-5 p 
in diameter. The sterigmata are of the usual form, 8-10 x 2 By 
directed parallel to the conidiophore-stalk. (Fig. 16). The conidia 
are spherical or subglobose, 2.5-3 p (a few 4 ») in diameter. No 
sclerotia have so far been observed. 
The naked-eye colour of the vegetation is approximately that of 
Penicillium, that is to say slightly darker and less grey-green than 
