T44 BETTS : THE FUNGI OF THE BEE-HIVE. 
subglobose, 240 x 200 p to 70 m (average about 140-180 p) in 
diameter. The asci are numerous, spherical or subglobose, 15-20 pt 
(average 17-18 x) in diameter; each ascus contains cight ascopores 
of the usual form (Fig. 12), 8 x 5 p. 
The naked-eve colour of the vegetation is at first white, then 
bluish-green, later of a dull (brownish) green. The perithecia are 
bright sulphur-yellow in colour. 
In some cultures a violet colouring matter made its appearance ; 
in others, the culture when viewed from behind appeared reddish- 
orange. These were evidently the phenomena described by Mangin 
(21, pp- 349-351) as occurring with some varieties of Aspergillus 
glaucus. He states that the violet coloration appears in neutral or 
slightly alkaline, the red in slightly acid cultures; and that the pig- 
ment concerned is very sensitive to variations in the acidity or 
Fig. 12.—Aspergillus glaucus. Ascus and ascospores. xX 1,400. 
alkalinity of the medium, so much so that alterations taking 
place during the growth of the fungus affect it. As my estimations 
of the acidity and alkalinity of media were only made roughly, with 
litmus paper, no conclusions can be drawn from the results of the 
cultures as to this last point. 
The conidiophore-stalk, sterigmata, and conidia survive heat- 
ing in sulphuric and hydrochloric acids, but the former dissolves the 
basal part of the conidiophore-stalk. The perithecium wall, and 
especially the ascospores, partially resist hydrochloric acid, but dis- 
appear entirely on heating in sulphuric acid. In hot concentrated 
solution of caustic potash, all parts except the asci and ascospores 
are more or less disorganized; the perithecium-wall is ultimately 
coloured red-brown. The conidia become resistant earlier than the 
conidiophore-stalk and sterigmata; for, if young specimens be heated 
in sulphuric acid, only the conidia remain. 
