250 SYNOPSIS OF VETERINARY 



DIRECTIONS FOR USING TUBERCULIN FOR THE 

 DIAGNOSIS OF TUBERCULOSIS. 



The febrile reaction in tuberculous cattle following the sub- 

 cutaneous injection of tuberculin begins from six to ten hours 

 after the injection, reaches the maximum 9 to 15 hours after the 

 injection, and returns to the normal 18 to 26 hours after the in- 

 jection. 



The reaction varies from 1 to 6 degrees above the average 

 normal temperature. In healthy subjects the quantity of Tuber- 

 culin injected is so small that the animal is not affected by it. 



In conducting the tests the following course is recommended 

 to those who wish to obtain the most accurate results. 



1. — Begin to take the rectal temperature at 6 A. M., and 

 take it every two hours thereafter until midnight, to get the 

 normal temperature of the individual and to note that no disease 

 exists. 



2. — Make the injection at midnight. 



3. — Begin to take the temperature next morning at 6 A. M., 

 and continue as on preceding day, to note the reactions. 



Where large herds are to be examined or time does not per- 

 mit of more detail : 



1. — Begin to take the temperature at 8 A. M. and continue 

 every two hours until 10 P. M. (omitting at 8 P. M.-if more con- 

 venient). Or, take the temperature three times — morning, noon 

 and evening, — before the injection. 



2. — Make the injection at 10 P. M. 



3. — Take the temperature next morning at 6 or 8 A. M., and 

 every two hours thereafter until 6 or 8 P. M. 



Inject 2 cubic centimeters (about 30 minims) of the tuber- 

 culin solution. Yearlings and two-year-olds should receive 1 to 

 iy 2 cubic centimeters, according to size. Bulls and very large 

 animals may receive 3 cubic centimeters. Make the injection be- 

 neath skin of neck or shoulder. 



If a second injection is made, increase the dose. 



There is now and then uneasiness, trembling and the more 

 frequent passage of soft dung. There may also be slight acceler- 

 ation of the pulse and of the breathing. 



A rise in temperature on the day following injection of 2 or 

 more degrees Fahrenheit above the maximum observed on. the 



