viii T^BLE OF CONTENTS 



Chapter II. Sterilization, Disinfection, Antisepsis, Food 



Preservation 



Definitions, 62; Physical sterilization, 62; Mechanical removal, 62; Desiccation, 63; 

 Light, 63; Cold, 64; Heat, 64; Electricity, 71; Chemical sterilization, 71 ; Soaps 

 71; Acids, 71; Alkalies, 73; Oxidizing agents, 73; Inorganic salts, 74; Organic 

 poisons, 76; Antiseptics and preservatives, 78; Physical, 79; Chemical, 79; 

 Testing of antiseptics and disinfectants, 80. 



Chapter III. Culture Media 



Definition, 83 ; Glass-ware, 83 ; The common media, 84 ; Nutrient broth, 84; Titra- 

 tion of media, 85; Gelatin, 90; Agar, 92; Modifications, 92; Sterilizable special 

 media, 93 ; Potato, 93; Milk, 94; Peptone solution, 94; Nitrate brot]i, 95; Blood- 

 serum, 9s; LoeiBer's blood-serum, 96; Eggs, 96; Dorset's egg, 97; Bread paste, 

 97; Media containing tmcooked protein, 97 ; Sterile blood, 97; Ascitic fluid, 99; 

 Sterilization, 100; Sterile tissue, 100; Blood-streaked agar, loi; Blood-agar, 

 loi; Broth containing tissues, loi; Ascitic-fluid agar, loi; Ascitic fluid with 

 tissue, 101; Other special media, 102. 



Chapter IV. Collection of Material for Bacteriological 



Study 



General considerations, 103 ; Sampling water and foods, 103 ; Material from the 

 body, 103 ; Sputum, 104; Urine, 104; Blood and transudates, 104; Cerebro-spinal 

 fluid, 105; Feces and intestinal juice, 105; Pus and exudates, 106; Material from 

 autopsies, 107. 



Chapter V. The Cultivation of Micro-organi?ms 



Avoidance of contamination, 108; Isolation of bacteria, 109; Plate cultures, no; 

 Roll tubes, 114; Streak method, 116; Tall-tube method, 116; Colonies, 117; 

 Pure cultures, 117; Stock cultures, 118; Regulation of temperature, 119; High 

 temperature incubator, 119; Gas-regulator, 120; Automatic safety-burner, 123; 

 Incubator room, 124; Prevention of drying, 124; Low-temperature incubator, 

 125; Cultivation of anaerobic bacteria, 128; Deep stab, 128; Veillon tall-tube 

 method, 129; Fermentation tube, 129; Removal of oxygen, 129; Hydrogen at- 

 mosphere, 130; Further methods, 134. 



Chapter VI. Methods of Animal Experimentation 



Value of animal experimentation, 135 ; Care of animals, 135 ; Holding for operation, 

 136; Inoculation, 137; Subcutaneous and intraperitoneal, 137; Intracranial, 

 137; Into circulating blood, 137; Other sites, 138; Subcutaneous application, 

 138; Alimentary and respiratory infection, 138; Collodion capsules,-i38 ; Obser- 

 vation of infected animals, 140 ; Post-mortem examination, 140. 



