MATERIAL FOR BACTERIOLOGICAL STUDY 



105 



tip to fit the ordinary Luer needle. The tube is sterilized by hot 

 air and the needles are sterilized by boiling in liquid paraffin 

 (albolene). By putting i c.c. of lo per cent sodium citrate into 

 the tube, the blood specimen may be kept fluid and transported 

 a considerable distance to the laboratory before inoculating 

 the culture media. 





m 



^h 



Pig. 39. — Taylor's tube for vein punctures. 



Cerebrospinal fluid is obtained by inserting a sterilized needle 

 (4 cm. long for children, 8-10 cm. long for adults, and with lumen 

 I mm.) in the median line in the back, so that it enters the spinal 

 canal between the second and' third, or between the third and 

 fourth, lumbar vertebrae. Aseptic technic is essential. The fluid 

 coming from the needle is received in a sterile tube. 

 ~ Feces from infants and young children are best collected by 

 means of a heavy glass tube closed and rounded off at the end, and 

 provided with a lateral opening near the closed end. This is 

 enclosed in a larger tube and sterilized. It is inserted well mto 

 the rec;4;um with aseptic technic and the entrance of fecal material 



