io6 



BACTERIOLOGY 



through the lateral opening is favored by gently moving the tube. 

 It is then withdrawn and replaced in its original container to be 

 transported to the laboratory. From adults the feces are passed 

 directly into a sterilized covered agateware basin without other 

 special apparatus. 



Intestinal jUice from the duodenum may 

 be obtained in infants^ by inserting a sterile 

 rubber catheter, closed below with a sterilized 

 gelatin capsule, through the esophagus and 

 stomach into the duodenum. The capsule 

 is then blown off by pressure from a sterile 

 syringe attached at the other end of the 

 catheter and the fluid contents of the duode- 

 num aspirated. In adults^ the Einhorn duo- 

 denal tube is employed., The tube is steri- 

 lized by boiling and the lower opening sealed 

 with a sterilized gelatin capsule and by 

 finally coating with shellac. The tube is in- 

 serted through the esophagus and is carried 

 through the pylorus by peristalsis. Ordi- 

 narily it is inserted in the evening. On the 



Pig. 40. — Two types of . ,, . . , , , °, 



instrument for obtaining lollowmg morning the Seal at the lower end 



t'ctriScareTami^:- '^ ^^"^^^^ ^y pressure of a sterile syringe 

 tion. (After Schmidt and attached to the free end of the tube and 

 the sample of juice aspirated. Intestinal 

 juice may be obtained from various levels in the jejunum also by 

 regulating the length of tube inserted. 



Pus and other exudates are best collected in sterile glass capil- 

 lary pipettes (see page 33). A sterilized cotton swab, made 

 by winding a pledget of absorbent cotton around the end of a stiff 

 wire, enclosing it in a test-tube and sterilizing it, is also useful, 

 especially when it is impossible or undesirable to employ the 

 glass tube. 



1 Hess: Journ. Infectious Diseases, July, 1912, Vol. XI, pp. 71-76. 



2 MacNeal and Chace: Arch. Int. Med., Aug., 1913, Vol. XU, pp. 178-197. 



