DESCRIPTIVE CHART 



FOR USE IN BACTERIOLOGICAL INSTRUCTION 



Recommended by the Committee on the Chart for Identification of Bacterial Species at the 191 7 meeting of the 

 SOCIETY OF AMERICAN BACTERIOLOGISTS 



H.J. Conn 

 H. A. Harding 

 I. J. Kligler 

 W. D. Frost 



M.J. PRUCHA 



K. N. Atkins 



Committee 



Name of organism. 



Source 



Date of isolation. . . 



Culture No. 



Invigoration of culture 



Medium used 



Temperature 



Length of each incubatr.'i- 



Date 



Series No., 



. .°C. Number of transfers. 

 days. 



MORPHOLOGY 



Note — Underscore required terms. 



Sketches 



Vegktative Ci-'XLS, Medium used 



temp age'. days.] 



Form, spheres, short rods, long rods, filaments, com- 

 mas, short spirals, long spirals, curved. 

 Arrangement, single, pairs, chains, fours, cubical 

 packets. 



Limits of Size Size of Majority 



^ Ends, rounded, truncate, concave 



i Capsules, present on 



How stained 



Sporangia, present, absent. Medium used 



temp age days. 



Form, elliptical, short rods, spindled, clavate, drum- 

 sticks. 



Limits of Size Size of Majority 



Endospores, present, absent. 



Location of Endospores, central, polar. 



Form, spherical, elliptical, elongated. 



Limits of Size 



Size of Majority 



Wall, thick, thin. 



Sporangium wall, adherent, not adherent. 



Motility 



In broth On agar 



Flagella, No Attachment, polar, bipolar. 



peritrichiate. How stained 



Irregular Forms. 



Present on in. . . .days at °C. 



Form spindled, cuneate, filamentous, branched. 



Staining Reactions. 



i; 10 watery fuchsin, gentian violet, carbol fuchsin 

 LoefHer's alkaline methylene blue., 

 Special Stains. 



Gram Acid fast 



Neisser 



Studied by , 



BRIEF CHARACTERIZATION 



Group No 



GROUP NUMBER 



As each of the determinations listed below is made, 

 check the proper figure. When complete place the entire 

 group number in the space above. 



Endospores produced 



Endospores not produced 



Aerobic (Strict) 



Facultative anaerobic 



Anaerobic (Strict) 



Gelatin liquefied 



Gelatin rot liquefied 



Acid and gas from dextrose 



Acid without gas from dextrose 



No acid from dextrose 



No growth with dextrose 



Acid and gas from lactose 



Acid without gas from lactose 



No acid from lactose 



No growth with lactose 



Acid and gas from saccharose 



Acid without gas from saccharose 



No acid from saccharose 



No growth with saccharose 



Nitrates reduced with evolution of gas 



Nitrates reduced without gas 



Nitrates not reduced 



Fluorescent 



Violet chromogens 



Blue chromogens 



Green chromogens 



Yellow chromogens 



Orange chromogens 



Red chromogens ^ , 



Brown chijomogens . , , 



Pink chromogens 



Noa-chromogenic 



Diastatic action on starch, strong 



Diastatic action on starch, feeble 



Diastatic action on starch, absent 



Acid and gas from glycerin 



Acid without gas from glycerin 



No acid from glycerin 



No growth with glycerin 



The genus according to the system of Migula is given 

 its proper symbol which precedes the number thus: 



Bacillus coli (Esch.) Mig. becomes B. 222.111202 



100. 

 200.0 



lO.O 



20.0 

 30.0 



1 .0 



2.0 



0.1 



0.2 



0.3 



0.4 



O.OI 



0.02 



0.03 



0.04 



O.OOI 



0.002 



0.003 



0.004 



O .0001 



0.0003 



0.0003 



.00001 



0.00002 



o . 00003 



0.00004 



. 00005 

 0.00006 

 0.00007 



0.00008 

 .00009 

 0.00000 



O .000001 

 .000002 



o .000003 



O .0000001 



.0000002 

 0.0000003 

 .0000004 



