Source.... 



Date of Revivif action. , 



Date of Isolation,.,.; Name 



..Determined: When? By whom ? 



Group No. . . 

 Culture No.. 



to 



^ 



DETAILED FEATURES 



Note — Underscore required'terms. Observe notes. 



Vegetative Cells, Medium used 



temp age days. 



Form.Xspheres.^short rods, long rods, filaments, com- 

 mas, short spirals, long spirals, spindled, cuneate, 

 clavate, curved. 



Arrangement, single, Pairs, chains, fours, cubical 

 packets. 



Limits of Size 



Size of Majority 



Ends, rounded, truncate, concave. 



(Orientation (grouping) 

 Chains (No. of elements) 

 Orientation of Chains, parallel, 

 irregular. 

 Postfission movements, loop forming, folding, snap- 

 ping, slipping. 



Sporangia, present, absent. Medium used 



temp age days. 



Form, elleptical, short rods, spindled, clavate, drum- 

 sticks. 

 Limits of Size Size of Majority 



(Orientation (grouping) 

 Chains (No. of elements) 

 Orientation of Chains, parallel, 

 irregular. 

 Endospores, present, absent. 



Location of Endospores, central, polar. 

 Form, spherical, elliptical, elongated. 



Limits of Size 



Size of Majority 



Wall, thick, thin. 



Sporangium wall, adherent, not adherent. 

 Germination, equatorial, oblique, polar, bipolar, 

 by stretching, by absorption of spore wall. 



Capsules, present on 



ZooGLOEA, Pseudozoogloea. 



Flagella, No Attachment, polar, bipolar, 



lophotrichiate, peritrichtate. How Stained 



Involution Forms, on in. . .days at °C. 



Staining Reactions. 



i:io watery fuchsin, gentian violet, carbol fuchsm, 

 Loeffler's alkaline methylene blue. 

 Special Stains. 



Gram Glycogen 



Fat Acid fast 



Neisser 



Metachromatic granules, sporogenous granules. 

 Nutrient Broth. 



Surface growth, ring, pellicle, flocculent, membran- 

 ous, none. 

 Clouding, slight, moderate, strong, transient, per- 

 sistent, none, fluid, turbid. 



Odor, absent, decided, resembling 



Sediment, compact, flocculent, granular, flaky, viscid 

 on agitation, abundant, scant, none. 

 Agar Stroke. 



Growth, invisible, scanty, moderate, abundant, none. 

 Form of growth filiform, echinulate, beaded, spread- 

 ing, plumose, arborescent, rhtzoid. 

 Elevation of growth, fiat, effuse, raised, convex. 

 Lustre, glistening, dull, cretaceous. 

 Topography, smooth, contoured, rugose, verrucose. 

 Optical Characters, opaque, translucent, opalescent, 

 iridescent. 



Chromogenesis Photogenic. Fluorescent. 



Odor, absent, decided, resembling 



Consistency, slimy, butyrous, viscid, membranous, 



coriaceous, brittle. 

 Medium, grayed, browned, reddened, blued, greened. 

 Agar Colonies. 



Growth, slow, rapid, temperature . 



Form, punctiform, circular, irregular, ameboid, 

 mycelioid, filamentous, rhizoid. 

 Surface, smooth, rough, concentrically ringed, 



radiate, striate. 

 Elevation, fiat, effuse, raised, convex, pulvinate, 



umbonate. 

 Edge, entire, undulate, lobate, erose, lacerate, 



fimbriate, fioccose, curled. 

 Internal structure, amorphous, finely-, coarsely- 

 granular, grumose, filamentous, fioccose, curled. 

 Gelatin Colonies. 



Growth, slow, rapid. 

 Form, punctiform, circular, irregular, ameboid, 

 mycelioid, filamentous, rhizoid. 

 Elevation, fiat, effuse, raised, convex, pulvinate, 



crateriform {liquefying) . 

 Edge, entire, undulate, lobate, erose, lacerate, 



fimbriate, filamentous, fioccose, curled. 

 Liquefaction, cup, saucer, spreading. 



I 



S ^ w w s 

 s 5 J o y 



a:? 

 o ^ 



O s 



Pi „ 



H G - 

 « U -5 



S < fe" 



P* l-H r^ vi 



O S 

 O^ 



Gelatin Stab. 



Growth, uniform, best at top, best at bottom. 



Line of puncture, filiform, beaded, papillate, villous, 



plumose, arborescent. 

 Liquefaction, none, crateriform, napiform, infundi- 



buliform, saccate, startiform; begins in d, 



complete in d. 



Depth of liquefaction in tube of lo mm. diameter, 



evenly inoculated at 20°C. for 30 days mm. 



Potato. 



Growth, scanty, moderate, abundant, transient, 



persistent. 

 Form of growth, filiform, echinulate, beaded, 



spreading, plumose, arborescent, rhizoid. 

 Elevation of growth, fiat, effuse, raised, convex. 

 Lustre, glistening, dull, cretaceous. 

 Topography, smooth, contoured, rugose, verrucose. 

 Chromogenesis Pigment in water 



insoluble, soluble; other solvents 



Odor, absent, decided, resembling 



Consistency, slimy, butyrous, viscid, membranous, 



coriaceous, brittle. 

 Medium grayed, browned, reddened, blued, greened. 



Potato Starch Jelly. 



Growth, scanty, copious, absent. 

 Diastasic action, absent, feeble, profound. 



Medium stained 



Dextrin, present, absent. 



Temperature Relations. 



Optimum temperature for growth 20°C.: or 37°C.: 



or °C. 



Maximum temperature for growth °C. 



Minimum temperature for growth °C. 



Cohn's Solution. 



Growth, copious, scanty, absent. 

 'Mediu.m fluorescent, nonfluorescent. 



Uschinsky's Solution. 



Growth, copious, scanty, absent. 

 Fluid, viscid, not viscid, 



Loeffler's Blood Serum. 



Stroke invisible, scanty, moderate, abundant. 



Form of growth, filiform, echinulate, beaded, 



spreading, plumose, arborescent, rhizoid. 

 Elevation of growth, fiat, effuse, raised, convex. 

 Lustre, glistening, dull, cretaceous. 

 Topography, smooth, contoured, rugose, verrucose. 



Chromogenesis 



Medium grayed, browned, reddened, blued, greened. 

 Liquefaction begins in d., complete in d. 



Milk. 



Clearing without coagulation. 

 Coagulation prompt, delayed, absent. 



Extrusion of whey begins in days. 



Peptonization begins on. . . .d., complete on d. 



Coagulum, slowly peptonized, rapidly peptonized. 



Reaction, id. . . . 2d 4d. . . . lod. — 2od 



Consistency, slimy, viscid, unchanged. 

 Medium bro'ivned, reddened, blued, greened. 



Litmus Milk. 



Acid, alkaline, acid then alkakine, no change. 

 Prompt reduction, no reduction, partial slow re- 

 duction. 



Indol Production, feeble, moderate, strong, absent. 



Hydrogen Sulphdie, feeble, moderate, strong, ab- 

 sent. 



Ammongia Production, feeble, moderate, strong, ab- 

 sent, masked by acids. 



Nitrate in Nitrate Broth. Reduced, not reduced. 



Presence of nitrites Ammonia 



Presence of nitrates free nitrogen 



Endospores produced 100. 

 Endospores not produced 200. 



Aerobic (Strict) 10. Facultative anaerobic 20. 

 Anaerobic (Strict) 30. 



Gelatin liquefied i. 

 Gelatin not liquefied 2. 



Acid and gas .1. Acid without gas .2. 

 No acid .3. No growth .4 with dextrose. 



Acid and gas .01. Acid without gas .02. 

 No acid .03. No growth .04 with lactose. 



Acid and gas .001. Acid without gas .002. 



No acid .003. No growth .004 with saccharose. 



Nitrates reduced with gas .0001. 

 Nitrates reduced without gas .0002. 

 Nitrates not reduced .0003. 



Fluorescent .00001. Violet, .00002. Blue .00003 

 Green .00004. Yellow .00005. Orange .00006. Red 

 .00007. Brown .00008. Pink .00009 Non-chromo- 

 genic .00000. 



Diastasic action on potato starch, strong .000001 

 Feeble .ooooo2. Absent .000003. 



Acid and gas .ooooooi. Acid without gas .0000002 

 No acid .0000003. No growth .0000004 with glycerin. 



I. Fermentation-tubes con- 

 taining peptone-water or 

 sugar-free bouillon. 



At °C, 



Gas production, in per cent 



(coz) 



Growth in closed arm 



Amount cf acid produced d. 



Amount of acid prod uced d. 

 Amount of acid produced d. 



Products of growth. 



Nitrogen. Obtained from peptone, asparagin, 

 glycocoll, urea, ammonia salts, nitrogen. 



Silicate Jelly (Fermi's Solution). 

 Growth, copious, scanty, absent. 



Medium stained 



Best media for long-continued growth 



Pathogenic to Animals. 



Insects, crustaceans, fishes, reptiles, birds, mice, 

 rats, guinea pigs, rabbits, dogs, cats, sheep, 



goals, cattle, horses, monkeys, man 



Serum reactions, agglutinins, bactericidins, opsonins, 



precipitins, complement deviation 



Products of growth, soluble toxins, endotoxins. 

 Pathogenic to Plants. 



Plants affected 



Quick test_for differential purposes. 



Loss of Virulence on Culture Media, prompt, 

 gradual, not observed in months. 



Acids Produced 



Alkalies Produced 



Alcohols Produced 



Ferments: peptase, tryptase, chymase, diastase, 

 invertase, pectase, cytase, tyrosinase, oxidase^ 

 Peroxidase, reductase, catalase, lipase, glucase, 

 galactase, etc 



Crytals formed: 



Toleration of Acidfe: Great, medium, slight. 



Acids tested 



Toleration of Alkalies: Great, medium, slight. 



Alkalies Tested 



Optimum reaction for growth in bouillon, stated 

 in terms of Fuller's scale 



Killed readily by drying: resistant to drying. 



Per cent killed by freezing (salt and crushed ice 

 or liquid air) 



Sunlight: Exposure on ice in thinly sown agar 

 plates: one-half plate covered (time 15 minutes), 

 sensitive, not sensitive. 



Per cent killed 



Vitality on culture media: brief, moderate, long. 



Thermal death-point (10 minutes exposure in nu- 

 trient broth when this is adapted to growth of 

 organism) °0. 



BRIEF CHARACTERIZATION 



Diameter over i/i 



Chains, filaments 



Endospores 



Capsules 



Zoogloea, Pseudozoogloea 



Motile 



Involution forms 



Gram's Stain 



Cloudy, turbid 



Ring 



Pellicle 



Sediment 



Shining 



Dull 



Wrinkled 



Chromogenic 



Round 



Proteus-like 



Rhizoid 



Filamentous 



Curled 





Surface-growth 



Needle-growth 



Moderate, absent 



Abundant 



Discolored 



Starch destroyed 



Grows at 37°C. 



Grows in Cohn's Sol. 



Grows in Uschinsky's Sol. 



Gelatin 



Blood-serum 



Casein 



Agar, mannan 



Acid curd 



Rennet curd 



Casein peptonized 



Indol. 



Hydrogen sulphide 



Ammonia 



Nitrates reduced 



Fluorescent 



Luminous 



Animal pathogen, epizoon 



Plant pathogen, epiphyte 



Soil 



Milk 



Fresh water 



Salt water 



Sewage 



Iron bacterium 



Sulphur bacterium 



